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CONFIRMATION BY CRYOELECTRON MICROSCOPY OF THE ABSENCE OF CRYSTALLIZATION USING A VITRIFICATION SOLUTION.

Author(s) : VALDEZ C. A.

Type of article: Article

Summary

CONTINUOUS CRYO-SCANNING ELECTRON MICROSCOPY (SEM) AND FREEZE REPLICA OBSERVATIONS WERE CARRIED OUT ON MOUSE BLASTOCYSTS USING A VITRIFICATION SOLUTION (VS1, 10% GLYCEROL + 20% PROPYLENE GLYCOL AND VS2, 25% GLYCEROL + 25% PROPYLENE GLYCOL). CRYO-SEM OBSERVATION OF BLASTOCYSTS USING A QUICK FREEZ ING SOLUTION (QFS, 3 M GLYCEROL + 0.25 M SUCROSE) WAS ALSO PERFORMED. EXAMINATION OF THE VS REVEALED THE ABSENCE OF BOTH INTRA-AND EXTRACELLULAR ICE CRYSTALS, WHILE THE EXAMINATION OF THE QFS SHOWED THE PRESENCE OF SMALL INTRA-AND EXTRACELLULAR ICE CRYSTALS. THE VITRIFIED BLASTOCYSTS SHOWED MARKED SHRINKAGE AS COMPARED TO THE QUICKLY FROZEN BLASTOCYST.

Details

  • Original title: CONFIRMATION BY CRYOELECTRON MICROSCOPY OF THE ABSENCE OF CRYSTALLIZATION USING A VITRIFICATION SOLUTION.
  • Record ID : 1991-1389
  • Languages: English
  • Source: Cryo-Letters - vol. 11 - n. 5
  • Publication date: 1990
  • Document available for consultation in the library of the IIR headquarters only.

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