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CRYOPRESERVATION OF MOUSE EMBRYOS AT 77 K (-196 DEG C) BY VITRIFICATION.

Author(s) : AGRAWAL K. P., POLGE C.

Type of article: Article

Summary

EMBRYOS (8-16 CELLS) WERE OBTAINED FROM RANDOM BRED ALBINO MICE (6-8 WEEKS OLD) THAT WERE INDUCED TO SUPEROVULATE. EMBRYOS WERE EXPOSED TO INTRACELLULAR CRYOPROTECTING MEDIUM (GLYCEROL 10%, 1-2 PROPANEDIOL 20% IN PHOSPHATE BUFFERED SALT SOLUTION) FOR 10 MIN AND THEN TRANSFERRED TO EXTRACELLULAR VITRIFICATION MEDIUM (25% GLYCEROL, 25% 1-2 PROPANEDIOL IN THE SALT SOLUTION). VITRIFICATION MEDIUM AND DILUENT WERE LOADED IN A STRAW AND IMMEDIATELY PLUNGED INTO LIQUID NITROGEN. AFTER THAWING THE CONTENTS OF THE STRAW WERE MIXED AND EMPTIED IN A PETRI DISH. AFTER 3 WASHINGS IN CULTURE MEDIUM THE EMBRYOS WERE KEPT IN CO2 INCUBATOR. IN 3-STEP DILUTION, EMBRYOS EXHIBITED SURVIVAL OF 20-23%.

Details

  • Original title: CRYOPRESERVATION OF MOUSE EMBRYOS AT 77 K (-196 DEG C) BY VITRIFICATION.
  • Record ID : 1990-1316
  • Languages: English
  • Source: Indian J. exp. Biol. - vol. 27 - n. 4
  • Publication date: 1989
  • Document available for consultation in the library of the IIR headquarters only.

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