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Cryobiology of mammalian gametes (sperm and egg) and germ plasm (embryos).

[In Japanese. / En japonais.]

Author(s) : UTSUMI K.

Type of article: Article

Summary

This article is undertaken to investigate the occurrence of osmotic shock, cell viability and membrane functional status of mammalian germ cells and gametes during cooling at chilling-temperature and subzero temperature. New topics related to the novel protocole of cell freezing and to the application of cryopreservation are discussed. For practical and field application, the simplification of methods is used to remove cryoprotectant from cryopreserved embryos. Using a novel variation of rapid cooling, vitrification method without ice formation either inside or outside the embryos was developed to successful cryopreserved embryos. Thus, as the powerful techniques of genom analysis, embryo cloning and molecular biology are focused on fundamental and applied aspects of embryology and reproductive biology, there are new problems regarding the cryobiology of gamete cells and embryos to be solved.

Details

  • Original title: [In Japanese. / En japonais.]
  • Record ID : 1997-1826
  • Languages: Japanese
  • Subject: General information
  • Source: Low Temperature Medicine - vol. 22 - n. 2
  • Publication date: 1996/06
  • Document available for consultation in the library of the IIR headquarters only.

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