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CRYOPRESERVATION OF OOCYTES USING DIRECTIONAL COOLING AND ANTIFREEZE GLYCOPROTEINS.

Author(s) : RUBINSKY B., ARAV A., DEVRIES A. L.

Type of article: Article

Summary

A NEW TECHNIQUE AND A SIMPLE APPARATUS WERE DEVELOPED FOR COOLING AND WARMING CELLS RAPIDLY, UNDER CONTROLLED THERMAL CONDITIONS. THE TECHNIQUE, WHICH USES THE CONCEPTOF DIRECTIONAL COOLING, WAS EVALUATED THROUGH ANALYTICAL STUDIES AND EXPERIMENTS WITH IMMATURE PIG OOCYTES. DURING ATTEMPTS TO DEVELOP OPTIMAL PROTOCOLS FOR PRESERVATION OF PIG OOCYTES BY VITRIFICATION IT WAS DISCOVERED THAT ANTIFREEZE GLYCOPROTEINS FROM ANTARCTIC NOTOTHENIID FISHES HAVE SPECIAL CRYOPROTECTIVE PROPERTIES AND DRAMATICALLY IMPROVE THE SURVIVAL AND THE MORPHOLOGICAL INTEGRITY OF PIG OOCYTES RAPIDLY COOLED TO CRYOGENIC TEMPERATURES.

Details

  • Original title: CRYOPRESERVATION OF OOCYTES USING DIRECTIONAL COOLING AND ANTIFREEZE GLYCOPROTEINS.
  • Record ID : 1992-0889
  • Languages: English
  • Source: Cryo-Letters - vol. 12 - n. 2
  • Publication date: 1991
  • Document available for consultation in the library of the IIR headquarters only.

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