Share

Cryopreservation of coronary endothelium and endothelial-mediated responses.

Author(s) : KU D. D., LIU Q., NORTON P., CAULFIELD J. B.

Type of article: Article

Summary

In contrast to partial preservation of vascular smooth muscle cell function, cryostorage of coronary arteries in DMSO and fetal calf serum resulted in a near complete preservation of the endothelium and its vasoregulatory function. This protection was observed in both animal and human epicardial conduit coronary arteries, as well as in dog intramyocardial resistance arteries. The authors cryostored also canine cardiac valves and evaluated the vasorelaxant effects of valvular endothelium-derived vasoactive factors using a bioassay system. As was observed with intact coronary arteries, a near complete preservation of valvular endothelium and its production of vasoactive factors was noted following the freeze and thaw procedures. Thus, cryopreservation may provide a useful and efficient storage method for the conservation of limited supplies of biological vascular tissues for graft transplantation and scientific research.

Details

  • Original title: Cryopreservation of coronary endothelium and endothelial-mediated responses.
  • Record ID : 1995-1221
  • Languages: English
  • Source: Cryobiology - vol. 31 - n. 1
  • Publication date: 1994/02

Links


See other articles in this issue (9)
See the source

Indexing