CRYOPRESERVATION OF EMBRYONIC AXES OF RECALCITRANT SEED: JUGLANS REGIO L CV FRANQUETTE.

Author(s) : BOUCAUD M. T. de

Type of article: Article

Summary

EMBRYONIC AXES WITH VARIABLE MOISTURE CONTENT EXCISED FROM WALNUT WERE STORED AFTER RAPID FREEZING IN LIQUID NITROGEN WITH OR WITHOUT CRYOPROTECTANT. THEY WERE THEN THAWED AND GROWN ON MODIFIED DRIVER AND KUNIYUKI'S WALNUT MEDIUM SUPPLEMENTED WITH BENZYLAMINOPURINE. A CRYOPROTECTIVE SOLUTION CONTAINING A HIGH CONCENTRATION 5 M 1,2-PROPANEDIOL AND 20 % SUCROSE ALLOWED A LARGE SURVIVAL AND REGROWTH RATE WITH A 20 % MOISTURE CONTENT.

Details

  • Original title: CRYOPRESERVATION OF EMBRYONIC AXES OF RECALCITRANT SEED: JUGLANS REGIO L CV FRANQUETTE.
  • Record ID : 1992-0637
  • Languages: English
  • Source: Cryo-Letters - vol. 12 - n. 3
  • Publication date: 1991
  • Document available for consultation in the library of the IIR headquarters only.

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