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Freezing of isolated thylakoid membranes in complex media. 11. The cryoprotective efficiency of combinations of bovine serum albumine (BSA) with low-molecular-weight cryoprotectants.

Author(s) : SANTARIUS K. A.

Type of article: Article

Summary

Combined use of BSA and ethylene glycol monomethyl ether (EGMME) on isolated spinach chloroplast membranes revealed that the rapidly permeating compound may act either as cryoprotectant or as cryosensitizer. During mild freezing, membrane protection was provided by a synergistic action of the cryoagents. This was also true at more severe freezing in the presence of very low concentrations of EGMME. In contrast, under moderate and severe freezing conditions, distinct amounts of EGMME diminished or abolished membrane preservation afforded by the protein. Thylakoid damage due to very large amounts of BSA in the unfrozen fraction was prevented by extremely high concentrations of EGMME. The various mechanisms of membrane protection and injury by interactions between these solutes are discussed.

Details

  • Original title: Freezing of isolated thylakoid membranes in complex media. 11. The cryoprotective efficiency of combinations of bovine serum albumine (BSA) with low-molecular-weight cryoprotectants.
  • Record ID : 1997-0836
  • Languages: English
  • Source: Cryo-Letters - vol. 17 - n. 1
  • Publication date: 1996/01
  • Document available for consultation in the library of the IIR headquarters only.

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