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Cryopreservation of fetal skin is improved by extracellular trehalose.

Author(s) : ERDAG G., EROGLU A., MORGAN J. R., et al.

Type of article: Article

Summary

The authors tested trehalose in combination with dimethylsulfoxide (DMSO) in the cryopreservation of human fetal skin and compared it with DMSO and glycerol, protocols that are routinely used by skin banks. The viability of fetal skin from four groups (fresh, and cryopreserved with glycerol, DMSO, or trehalose/DMSO) were evaluated using an in vitro membrane assay and by transplantation to immunodeficient mice. The membrane integrity assay showed a 90% integrity in fresh, unfrozen fetal skin. The number of intact cells dropped to 23 and 44% in fetal skin cryopreserved with glycerol and DMSO, respectively. When trehalose was added to the cryopreservation medium containing DMSO, the membrane integrity rose to 65%. The results suggest that cryopreservation protocols routinely used by skin banks can be improved by combining sugars such as trehalose with a permeating cryoprotectant.

Details

  • Original title: Cryopreservation of fetal skin is improved by extracellular trehalose.
  • Record ID : 2003-2051
  • Languages: English
  • Source: Cryobiology - vol. 44 - n. 3
  • Publication date: 2002/06

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