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Cryopreservation of grape embryogenic cell suspensions. 2. Influence of post-thaw culture conditions and application to different strains.

Author(s) : DUSSERT S., MAURO M. C., ENGELMANN F.

Type of article: Article

Summary

In the work, the influence of post-thaw culture conditions on the survival and recovery of a grape embryogenic cell suspension was investigated. Growth recovery was maximal with 1 mg/l naphtoxyacetic acid. The presence of activated charcoal reduced the regrowth of cryopreserved cells. A minimal 6-day culture on semi-solid medium was necessary in order to obtain regrowth of cryopreserved cells in liquid medium. Embryos obtained from frozen cultures germinated normally in vitro. This technique was successfully applied to 3 additional embryogenic strains.

Details

  • Original title: Cryopreservation of grape embryogenic cell suspensions. 2. Influence of post-thaw culture conditions and application to different strains.
  • Record ID : 1993-0262
  • Languages: English
  • Source: Cryo-Letters - vol. 13 - n. 1
  • Publication date: 1992/01
  • Document available for consultation in the library of the IIR headquarters only.

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