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Cryopreservation of grape embryogenic cell suspensions: 1) influence of pretreatment, freezing and thawing conditions.

Author(s) : DUSSERT S., et al.

Type of article: Article

Summary

During this work, conditions allowing for the cryopreservation of an embryogenic grape cell suspension were defined. Survival rates higher than 60% could be obtained with cells at 30% PCV (Packed Cell Volume) after 1-hour pretreatment at 0 deg C with 0.25 M maltose and 5% DMSO, freezing at 0.5 deg C/min to -40 deg C followed by immersion in liquid nitrogen. After rapid thawing, the cells were allowed to grow for 18 days on a semi-solid medium containing activated charcoal. The cells were then transferred to liquid medium. One and a half months after thawing, the growth of the cryopreserved cells was similar to that of the unfrozen control.

Details

  • Original title: Cryopreservation of grape embryogenic cell suspensions: 1) influence of pretreatment, freezing and thawing conditions.
  • Record ID : 1993-0264
  • Languages: English
  • Source: Cryo-Letters - vol. 12 - n. 5
  • Publication date: 1991/09
  • Document available for consultation in the library of the IIR headquarters only.

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