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CRYOPRESERVATION OF HUMAN BONE MARROW CELLS BY A MODIFICATION OF THE TWO-STEP COOLING TEMPERATURE.

Author(s) : BERTHIER R.

Type of article: Article

Summary

A REPORT ON THE FIRST ATTEMPT TO FREEZE HUMAN BONE MARROW CELLS WITH A TWO-STEP COOLING METHOD AND A SIMPLE AND RELIABLE WAY OF OBTAINING STABLE FIRST-STEP SUBZERO FREEZING BATHS. THE OPTIMAL FREEZING TEMPERATURE WAS FOUND TO BE IN THE RANGE OF 237 TO 235.5 K (-36 TO -37.5 DEG C) FOR BONE MARROW PROGENITOR CELL SURVIVAL. SUCH TEMPERATURES WERE EASILY OBTAINED IN STABLE SLUDGES OF ANISOLE OR K2CO3 EUTECTIC SOLUTION IN WATER. THE OPTIMAL HOLDING TIME WAS 20 MIN BEFORE PLUNGING TUBES INTO LIQUID NITROGEN.

Details

  • Original title: CRYOPRESERVATION OF HUMAN BONE MARROW CELLS BY A MODIFICATION OF THE TWO-STEP COOLING TEMPERATURE.
  • Record ID : 1984-2121
  • Languages: English
  • Source: Cryobiology - vol. 20 - n. 6
  • Publication date: 1983

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