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Modification of the state of bone marrow hematopoietic cells after cryopreservation.

Author(s) : GOLTSEV A. N., BABENKO N. N., DUBRAVA T. G., et al.

Type of article: Article, IJR article

Summary

The structural and functional characteristics of bone marrow (BM) hematopoietic stem cells (HSCs) prior to and after cryopreservation were studied using different methodical approaches. The HSCs colony-forming ability and the cell capability to homing and proliferation were analyzed. The authors observed a temporary decrease in capability of the cells in detecting hematopoietic microenvironment and to be settled in spleen and BM (homing) of the mouse after cryopreservation and following transplantation. The part of cryopreserved cells settled after transplantation lost the capability of proliferating. To check possible transformations in cell receptor repertoire, the authors have estimated the Thy-1,2-antigen presence on HSCs and the dynamics of its synthesis after immune modulation. The decreased accumulation of the antigen in HSCs was contributed, at least partially, to shedding this structure off the cell membranes after cryopreservation. The damaging effect of low temperature was detected at all levels of cell metabolism regulation: from signal perception by all HSCs receptors, its transduction, and realization at transcription and translation levels.

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Pages: 358-367

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Details

  • Original title: Modification of the state of bone marrow hematopoietic cells after cryopreservation.
  • Record ID : 2006-1507
  • Languages: English
  • Source: International Journal of Refrigeration - Revue Internationale du Froid - vol. 29 - n. 3
  • Publication date: 2006/05

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