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CRYOPRESERVATION OF SPINACH CHLOROPLAST MEMBRANES BY LOW-MOLECULAR-WEIGHT CARBOHYDRATES. II. DISCRIMINATION BETWEEN COLLIGATIVE AND NONCOLLIGATIVE PROTECTION.

Author(s) : SANTARIUS K. A., GIERSCH C.

Type of article: Article

Summary

THYLAKOID MEMBRANES ISOLATED FROM SPINACH LEAVES (SPINACIA OLERACEA L. CV MONATOL) WERE SUBJECTED TO A FREEZE-THAW CYCLE IN THE PRESENCE OF VARIOUS CONCENTRATIONS OF SUGARS, POLYHYDRIC ALCOHOLS, AND SODIUM CHLORIDE. CRYOPRESERVATION BY LOW-MOLECULAR-WEIGHT COMPOUNDS IS PREDOMINANTLY DUE TO COLLIGATIVE ACTION OF THE SOLUTES. AT SODIUM CHLORIDE CONCENTRATIONS EXCEEDING 15 MILLIMOLES THE RELATIVE CONTRIBUTION OF NONCOLLIGATIVE MEMBRANE PROTECTION WAS INDEPENDENT OF THE CONCENTRATION. HOWEVER, DURING FREEZING IN THE PRESENCE OF SALT, FOR INSTANCE 1-4 MILLIMOLES SODIUM CHLORIDE, CRYOPROTECTION DUE TO COLLIGATIVE PHENOMENA IS REDUCED IN FAVOUR OF OTHER MECHANISMS.

Details

  • Original title: CRYOPRESERVATION OF SPINACH CHLOROPLAST MEMBRANES BY LOW-MOLECULAR-WEIGHT CARBOHYDRATES. II. DISCRIMINATION BETWEEN COLLIGATIVE AND NONCOLLIGATIVE PROTECTION.
  • Record ID : 1983-1996
  • Languages: English
  • Source: Cryobiology - vol. 20 - n. 1
  • Publication date: 1983

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