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Cryopreservation of human ovarian tissue by direct plunging into liquid nitrogen: negative effect of disaccharides in vitrification solution.

Author(s) : ISACHENKO V., ISACHENKO E., RAHIMI G., et al.

Type of article: Article

Summary

Previous successful attempts to cryopreserve human ovarian tissue by direct plunging into liquid nitrogen gave rise to the study designed to establish a future research direction. Bovine oviductal epithelial fragments (as a tissue model) and large biopsy fragments of human ovarian tissue were used for cryopreservation. Two protocols were tested: with permeable cryoprotectants (DMSO, propylene glycol, glycerol) + egg yolk + sucrose or trehalose + a synthetic blocker of ice nucleation, Supercool X-1000; and using only permeable cryoprotectants (glycerol and ethylene glycol) + egg yolk + Supercool X-1000. The cryopreserved tissue specimens were subsequently thawed and the cryoprotectants removed. Both the dynamic growth and hormonal activity of the ovarian tissue, vitrified using only permeable cryoprotectants, were greater than after vitrification in a mixture of permeable cryoprotectants and sucrose. Unlike findings using other reproductive tissue (spermatozoa, oocytes, embryos), these results suggest that the cryopreservation of ovarian tissue by direct plunging into liquid nitrogen must be achieved by vitrification using only permeable cryoprotectants and agents that prevent ice formation.

Details

  • Original title: Cryopreservation of human ovarian tissue by direct plunging into liquid nitrogen: negative effect of disaccharides in vitrification solution.
  • Record ID : 2003-3030
  • Languages: English
  • Source: CryoLetters - vol. 23 - n. 5
  • Publication date: 2002/09
  • Document available for consultation in the library of the IIR headquarters only.

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