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CRYOPRESERVATION OF HUMAN PERIPHERAL BLOOD STEM CELLS: OPTIMAL COOLING AND WARMING CONDITIONS.

Author(s) : MCGANN L. E.

Type of article: Article

Summary

PERIPHERAL BLOOD STEM CELLS ARE BEING USED TO RECONSTITUTE HUMAN BONE MARROW FUNCTION AFTER ABLATIVE THERAPY OF BLAST TRANSFORMATION OF CHRONIC MYELOGENOUS LEUKEMIA. STUDIES WERE UNDERTAKEN TO ESTABLISH THE OPTIMUM COOLING AND WARMING CONDITIONS OF THE PRESERVATION OF COLONY-FORMING ACTIVITY IN THE PERIPHERAL BLOOD OF PATIENTS WITH CML. THE RESULTS SHOW THAT MAXIMUM RECOVERY OF CFU-C ACTIVITY OCCURS AFTER COOLING AT 3K/MIN, AN AVERAGE OF 50% BETTER THAN THE RECOVERY FOLLOWING COOLING AT 1K/MIN. CFU-C RECOVERY DECREASED WITH DECREASING WARMING RATE, BUT HIGH RECOVERY WAS OBTAINED WITH WARMING RATES AS LOW AS 10K/MIN. VIABLE CELL COUNT DID NOT CORRELATE WITH CFU-C RECOVERY, THEREFORE IT REPRESENTS A POOR INDEX FOR QUALITY CONTROL.

Details

  • Original title: CRYOPRESERVATION OF HUMAN PERIPHERAL BLOOD STEM CELLS: OPTIMAL COOLING AND WARMING CONDITIONS.
  • Record ID : 1982-1757
  • Languages: English
  • Source: Cryobiology - vol. 18 - n. 5
  • Publication date: 1981

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