Cryopreservation of microspore embryos of oilseed rape (Brassica napus L.) by dehydration in air with or without alginate encapsulation.

Author(s) : URAGAMI A., LUCAS M. O., RALAMBOSOA J., RENARD M., DEREUDDRE J.

Type of article: Article

Summary

Two-week-old microspore embryos of oilseed rape (Brassica napus L. cv Topas) were successfully cryopreserved after calcium-alginate encapsulation, high-sucrose preculture and air dehydration. Cryopreservation was also successful without encapsulation although plantlet recovery rates were lower than that after encapsulation-dehydration. After encapsulation, recovery rates were higher when embryos were extracted from the beads one day after rewarming.

Details

Original title: Cryopreservation of microspore embryos of oilseed rape (Brassica napus L.) by dehydration in air with or without alginate encapsulation.
Record ID : 1994-0933
Languages: English
Source: Cryo-Letters - vol. 14 - n. 2
Publication date: 1993/03
Document available for consultation in the library of the IIR headquarters only.

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Indexing

Themes: Seeds and plants
Keywords: Rapeseed; Cryoprotectant; Coating (food); Survival; Seed; Embryo; Dehydration; Cryopreservation