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SURVIVAL OF MOUSE EMBRYOS AFTER BEING FROZEN IN GLYCEROL-SUCROSE MIXTURE.

Author(s) : AGRAWAL K. P., POLGE C.

Type of article: Article

Summary

190 EMBRYOS (8 TO 16 CELLS) WERE DEHYDRATED IN GLYCEROL-SUCROSE MIXTURE IN 0.25 ML STRAWS AT ROOM TEMPERATURE. STRAWS WERE COOLED AT THE RATE OF 5 K/MIN TO 266 K (-7 DEG C). STRAWS WERE FURTHER COOLED AT 0.5 K/MIN DOWN TO 238 K (-35 DEG C) AND THEN PLUNGED INTO LIQUID NITROGEN. THAWING OF STRAWS WAS DONE BY DIRECT TRANSFER INTO WATER AT 308 K (35 DEG C).169 EMBRYOS WERE RECOVERED ON THAWING. 93.5% WERE APPARENTLY NORMAL AND USED FOR CULTURE. 47.46% DEVELOPED TO MORULAE/EARLY BLASTOCYSTS AND 45.56% TO EXPANDED BLASTOCYSTS ON 24 AND 48 HOUR CULTURE, RESPECTIVELY. IN CONCLUSION, THE INCORPORATION OF SUCROSE IN THE FREEZING MEDIUM AT 0.25 M HAS LED TO PROPOSE A FREEZING, THAWING AND TRANSFER METHOD WITHOUT DILUTION OF GLYCEROL.

Details

  • Original title: SURVIVAL OF MOUSE EMBRYOS AFTER BEING FROZEN IN GLYCEROL-SUCROSE MIXTURE.
  • Record ID : 1990-1315
  • Languages: English
  • Source: Indian J. exp. Biol. - vol. 37 - n. 5
  • Publication date: 1989
  • Document available for consultation in the library of the IIR headquarters only.

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