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Cryopreservation of the Australian species Macropidia fuliginosa (Haemodoraceae) by vitrification.

Author(s) : TURNER S. R., TAN B., SENARATNA T., et al.

Type of article: Article

Summary

Somatic embryos were used to develop a cryopreservation protocol for Macropidia fuliginosa, a commercially-important species endemic to the southwest of Western Australia. Somatic embryos were allowed to develop from embryogenic callus for three weeks on an kinetin medium prior to processing. These were transferred and cultured on a solid agar medium supplemented with 0 to 0.6 M sorbitol for 2 d prior to incubation in the plant vitrification solution two (PVS2). The embryos were then washed in 1 M sucrose solution (treated controls) or cooled in liquid nitrogen. Highest survival for cooled treatments (67.3%) was achieved by preculture with 0.4 M sorbitol, then incubation in PVS2. Further experimentation varying pre-culture duration (2 or 3 d) and incubation on either glycerol (0.8 M) or sorbitol (0.4 M) indicated that very high survival (90.6%) of embryos was achievable by adopting a 2 d preculture period on 0.8 M glycerol. The phenotype and growth rates of plants obtained were similar to those of parents plants. This optimised procedure was then applied to tissue culture-derived shoot apices of the same clone and resulted in a high survival rate (84.4%).

Details

  • Original title: Cryopreservation of the Australian species Macropidia fuliginosa (Haemodoraceae) by vitrification.
  • Record ID : 2002-0260
  • Languages: English
  • Source: CryoLetters - vol. 21 - n. 6
  • Publication date: 2000/11
  • Document available for consultation in the library of the IIR headquarters only.

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