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Cryopreservation of pancreatic islet cells.

[In Japanese. / En japonais.]

Author(s) : YOKOGAWA Y., ONO J.

Type of article: Article

Summary

Single cells dispersed from isolated islets of Syrian golden hamsters were frozen in various conditions with a program freezer. DMSO and glycerol at a concentration of 10% were also determined for their effect as a cryoprotective agent. Islets were harvested from Syrian golden hamster by collagenase digestion, and dispersed by EDTA-dispase treatment. Islet cells of 1,000,000 were suspended in the medium with 10% DMSO as a cryoprotective agent. The survival rate of islet cells after freezing and thawing was highest when they were cooled at a rate of 0.5 deg C/minute down to -40 deg C followed by 5 deg C/minute to -70 deg C in DMSO containing medium.

Details

  • Original title: [In Japanese. / En japonais.]
  • Record ID : 1996-3746
  • Languages: Japanese
  • Source: Low Temperature Medicine - vol. 22 - n. 1
  • Publication date: 1996/01
  • Document available for consultation in the library of the IIR headquarters only.

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