CRYOPRESERVATION OF MOUSE, MONKEY AND HUMAN ISLETS OF LANGERHANS FOR TRANSPLANTATION PURPOSE.

[In Russian. / En russe.]

Author(s) : JUTTE N. H. P. M.

Type of article: Article

Summary

INSTEAD OF AN ENTIRE PANCREAS, ISOLATED ISLETS OF LANGERHANS CAN BE TREATED PRIOR TO TRANSPLANTATIONTO REDUCE THEIR IMMUNOGENICITY. THUS, THE SUCCESS RATE IN ALLOTRANSPLANTATION OF ISLETS CAN BE INCREASED. THE ISOLATION OF LARGE AMOUNTS OF ISLETS FROM THE HUMAN PANCREAS IS STILL A PROBLEM, AND ISLETS FROM DIFFERENT DONORS HAVE TO BE COLLECTED AND STORED BY CRYOPRESERVATION. IN THE PAPER THE AUTHORS DESCRIBE THE METHODS USED IN THEIR LABORATORY TO ISOLATE ISLETS OF LANGERHANS FROM RODENT, MONKEY AND HUMAN PANCREASES. THE RESULTS OF SUCCESSFUL CRYOPRESERVATION OF THESE ISLETS ARE PRESENTED. FIRST, BY A MORE CONVENTIONAL FREEZING METHOD USING DMSO AS CRYOPROTECTANT. SECOND, BY VITRIFICATION USING A MIXTURE OF CRYOPROTECTANTS.

Details

  • Original title: [In Russian. / En russe.]
  • Record ID : 1992-0893
  • Languages: Russian
  • Source: Probl. Kriobiol. - n. 1
  • Publication date: 1991
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (5)
See the source