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DISPERSED PANCREATIC GRAFT CRYOPRESERVATION IN THE DOG: IN VIVO ASSESSMENT OF PRESERVATION PROTOCOLS.

Author(s) : WALSH T. N., ALDERSON D., FARNDON J. R.

Type of article: Article

Summary

DISPERSED CANINE PANCREATIC GRAFTS WERE CRYOPRESERVED AND THE IN VIVO FUNCTION WAS STUDIED FOLLOWING INTRASPLENIC AUTOTRANSPLANTATION. 4 PROTOCOLS WERE EMPLOYED, EXAMINING THE EFFECTS OF COOLING AND THAWING RATES AND DMSO CONCENTRATION ON GRAFT SURVIVAL. COOLING AT 0.08 K/SEC AND THAWING AT 1.3 K/SEC ALLOWED 3 SUCCESSFUL GRAFTS FROM 7 WHEN THAWED AT 1.3 K/SEC USING 1.4 OR 2 M DMSO BUT ONLY ONE SUCCESS FROM EIGHT WHEN THAWED AT 0.13 K/SEC. OF THE 7 EXPERIMENTS WHERE SUCCESSFUL PRESERVATION WAS ACHIEVED GRAFT INJURY WAS ESTIMATED AS LESS THAN 50% IN 4 BUT FOR 3 IT WAS PROBABLY GREATER THAN 50%. SUCCESSFUL GRAFT IMPLANTATION DOES NOT GUARANTEE INDEFINITE SURVIVAL AS 6 OF 7 GRAFTS IN THE STUDY BECAME EXHAUSTED WITHIN 13 MONTHS OF IMPLANTATION.

Details

  • Original title: DISPERSED PANCREATIC GRAFT CRYOPRESERVATION IN THE DOG: IN VIVO ASSESSMENT OF PRESERVATION PROTOCOLS.
  • Record ID : 1989-0357
  • Languages: English
  • Source: Cryobiology - vol. 24 - n. 3
  • Publication date: 1987

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