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Successful fertility experiments with cryopreserved spermatozoa of barramundi, Lates calcarifer (Bloch), using dimethylsulfoxide and glycerol as cryoprotectants.

Author(s) : PALMER P. J., BLACKSHAW A. W., GARRETT R. N.

Type of article: Article

Summary

The fertility of cryopreserved Lates calcarifer sperm was studied to increase the availability of semen for routine fertilization of stripped eggs and to provide a tool for selective breeding. Semen diluted (1:4 v/v) and frozen (-196 deg C) with 5% DMSO or 10% glycerol (final concentration) as cryoprotectants was used to inseminate freshly stripped ova. Sperm held in liquid nitrogen for 90 days with DMSO yielded acceptable fertilization and hatching rates with semen-to-ova ratios of up to 1:100 (v/v), and produced fish with no apparent abnormalities over a 29-day period after hatch.

Details

  • Original title: Successful fertility experiments with cryopreserved spermatozoa of barramundi, Lates calcarifer (Bloch), using dimethylsulfoxide and glycerol as cryoprotectants.
  • Record ID : 1994-1902
  • Languages: English
  • Source: Reprod. Fertil. Dev. - vol. 5 - n. 3
  • Publication date: 1993
  • Document available for consultation in the library of the IIR headquarters only.

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