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Cryopreservation of spermatozoa from freeze-tolerant and -intolerant anurans.

Author(s) : BEESLEY S. G., COSTANZO J. P., LEE R. E. Jr

Type of article: Article

Summary

Spermatozoa of the freeze-tolerant wood frog (Rana sylvatica) were used to develop a general protocol for the frozen storage of amphibian spermatozoa. The authors tested the efficacy of four cryoprotectants (DMSO, methanol, glycerol, and ethylene glycol), two supplements (fetal bovine serum or glutathione), and combinations of these cryoprotectants and supplements. DMSO and fetal bovine serum were the most effective cryoprotectant and supplement, respectively, in reducing sperm lysis. Vital dye assays showed that viability was greatest for spermatozoa treated with both DMSO and fetal bovine serum. Thus, this combination was used to cryopreserve spermatozoa from the freeze-intolerant anurans, Rana pipiens and Bufo americanus. Recovery of viable spermatozoa was significantly greater for R. sylvatica than for R. pipiens and B. americanus. Nonetheless, the results support the feasibility of using gamete cryopreservation techniques in programs aimed at the captive propagation of amphibians.

Details

  • Original title: Cryopreservation of spermatozoa from freeze-tolerant and -intolerant anurans.
  • Record ID : 1999-2573
  • Languages: English
  • Source: Cryobiology - vol. 37 - n. 2
  • Publication date: 1998/09

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