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Cryoprotectant permeability parameters for cells used in a bioengineered human corneal equivalent and applications for cryopreservation.

Author(s) : EBERTZ S. L., MCGANN L. E.

Type of article: Article

Summary

A human corneal equivalent is being developed with applications in pharmaceutical testing and biomedical research, but the distribution of this engineered tissue, depends on successful cryopreservation. Cryopreservation of tissues depends on the presence of cryoprotectants, their addition and removal, and exposure to conditions during freezing and thawing, all of which depend on cellular membrane permeabilities to water and cryoprotectant. This study defines the permeability properties that define the rate of water and cryoprotectant movement across the plasma membrane of isolated human corneal endothelial, keratocyte, and epithelial cells.

Details

  • Original title: Cryoprotectant permeability parameters for cells used in a bioengineered human corneal equivalent and applications for cryopreservation.
  • Record ID : 2005-1496
  • Languages: English
  • Source: Cryobiology - vol. 49 - n. 2
  • Publication date: 2004/10

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