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Corneal cryopreservation with chondroitin sulfate.

Author(s) : HAGENAH M., BOHNKE M.

Type of article: Article

Summary

In an experimental study using porcine corneas the authors investigated the influence of several variables of freeze-thaw trauma on survival of corneal endothelial cells after corneal cryopreservation employing chondroitin sulfate as a cryoprotectant. The authors examined the influence of: 1) the concentration of chondroitin sulfate in the cryopreservation medium; 2) the concentration of fetal calf serum in the cryopreservation medium; 3) the cooling rate; and 4) the preincubation period in the cryopreservation medium before freezing. Corneal cryopreservation with chondroitin sulfate provided higher corneal endothelial cell densities than corneas preserved by conventional methods. Moreover, even slight changes in variables that affect the tissue in the freeze-thaw cycle have a major impact on corneal endothelial cell survival after cryopreservation.

Details

  • Original title: Corneal cryopreservation with chondroitin sulfate.
  • Record ID : 1994-1896
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 4
  • Publication date: 1993/08

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