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CRYOPROTECTANTS AS KOSMOTROPIC HOFMEISTER SOLUTES.

Author(s) : KOYNOVA R. D., TENCHOV B. G.

Summary

THE AUTHORS PRESENT CALORIMETRIC DATA FOR FULLY HYDRATED PHOSPHOLIPIDS AND GLYCOLIPIDS. THEY SEEK TO DEMONSTRATE THAT THE CRYOPROTECTANT EFFECTS OF VARIOUS SUBSTANCES IN THE PRESENCE OF WATER MIGHT ALSO INVOLVE INDIRECT (HOFMEISTER) INTER-ACTIONS WITH THE FULLY HYDRATED MEMBRANES. THEY FIND THAT KOSMOTROPIC SOLUTES (SUGARS AND POSSIBLY OTHER CRYOPROTECTANTS) STABILIZE THE STRUCTURE OF BULK WATER AND THUS FAVOUR SMALLER LIPID HEADGROUP AREAS. THIS LEADS IN PARTICULAR TO A PREFERENCE FOR THE INVERTED HEXAGONAL PHASE OVER THE LAMELLAR LIQUID CRYSTALLINE PHASE AND TO A DECREASE OF THE TRANSITION TEMPERATURE BETWEEN THE 2 PHASES. THE KOSMOTROPIC PROPERTIES OF THE CRYOPROTECTANTS CAN BE RESPONSIBLE ALSO FOR THE CONSERVATION OF THE NATIVE CONFORMATION OF PROTEINS IN SOLUTIONS THAT ACT AGAINST PERTURBATIONS SUCH AS THERMALLY INDUCED UNFOLDING AND PH-INDUCED DISSOCIATION. M. C.

Details

  • Original title: CRYOPROTECTANTS AS KOSMOTROPIC HOFMEISTER SOLUTES.
  • Record ID : 1990-2130
  • Languages: English
  • Publication date: 1989/07/06
  • Source: Source: Proc. IVth int. Sch. Cryobiol. Freeze-drying, Borovets
    L55-L56; 3 fig.; 1 tabl.; 14 ref.
  • Document available for consultation in the library of the IIR headquarters only.

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