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Cryoprotection of red blood cells by a 2,3-butanediol containing mainly the levo and dextro isomers.

Author(s) : BOUTRON P.

Type of article: Article

Summary

A 2,3-butanediol containing 96.7% racemic mixture of the levo and dextro isomers and only 3.1% of the meso isomer (called 2,3-butanediol 97% dl) has been used for the cryoprotection of red blood cells. The erythrocytes were cooled to -196 deg C at rates between 2 and 3500 deg C/min, followed by slow or rapid warming. Various results, in terms of percentage of survival cells are given. In agreement with the remarkable physical properties of its aqueous solutions, the present experiments also suggest that 2,3-butanediol containing mainly the levo and dextro isomers could be a very useful cryoprotectant for organ cryopreservation. However, it would perhaps be better to use it in combination with other cryoprotectants, since it is a little more toxic than glycerol or 1,2-propanediol at high concentrations.

Details

  • Original title: Cryoprotection of red blood cells by a 2,3-butanediol containing mainly the levo and dextro isomers.
  • Record ID : 1993-1132
  • Languages: English
  • Source: Cryobiology - vol. 29 - n. 3
  • Publication date: 1992/06

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