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Effect of phosphoenolpyruvate on metabolic and morphological recovery of red cells after prolonged liquid storage and subsequent freezing in glycerol medium.

Author(s) : OHYAMA M., ARITAKE H., SHIRAKI H., et al.

Type of article: Article

Summary

The study was designed to determine the effects of phosphoenolpyruvate (PEP) treatment of red blood cells (RBCs) previously cold stored for a prolonged period in a liquid medium and the freezing of these treated cells in glycerol. RBCs stored for 21 days at 4 deg C were incubated for 30 minutes at 37 deg C with rejuvenant solution containing 50 milliM PEP, 60 milliM mannitol, 30 milliM sodium chloride, 25 milliM glucose, and 1 milliM adenine, pH 6.0, and then frozen at -80 deg C for 4 weeks. The results indicate that maximum recovery of viable RBCs can be attained when frozen and thawed red cells are processed from cells stored in the frozen state after they had been rejuvenated with PEP even after prolonged liquid storage.

Details

  • Original title: Effect of phosphoenolpyruvate on metabolic and morphological recovery of red cells after prolonged liquid storage and subsequent freezing in glycerol medium.
  • Record ID : 1993-1131
  • Languages: English
  • Source: Cryobiology - vol. 29 - n. 3
  • Publication date: 1992/06

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