Share

CRYOSCANNING ELECTRON MICROSCOPY OF ICE CRYSTAL DAMAGE IN RAPIDLY FROZEN, CRYOMOUNTED SPECIMENS.

Author(s) : RYAN K. P.

Type of article: Article

Summary

THIN ARROW-WORM SPECIMENS SHOWED GOOD CRYOFIXATION WITH NO ICE CRYSTALS BEING OBSERVABLE WITHIN 25 MICROMETERS OF THE SURFACE, AFTER FREEZING IN LIQUID ETHANE AND MOUNTING WITH LOW TEMPERATURE CEMENTS. CARROT SPECIMENS, REGARDLESS OF CRYOPREPARATION METHOD, SHOWED SEVERE DAMAGE 1 MM FROM THE SURFACE. THIS SUGGESTS THAT THERE IS NO ADVANTAGE IN USING SPECIAL COOLANTS FOR CRYOSCANNING ELECTRON MICROSCOPY, UNLESS SMALL SPECIMENS ARE TO BE FROZEN AND FRACTURED FOR INTERNAL EXAMINATION.

Details

  • Original title: CRYOSCANNING ELECTRON MICROSCOPY OF ICE CRYSTAL DAMAGE IN RAPIDLY FROZEN, CRYOMOUNTED SPECIMENS.
  • Record ID : 1989-1611
  • Languages: English
  • Source: Cryo-Letters - vol. 9 - n. 6
  • Publication date: 1988
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (1)
See the source

Indexing