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Desiccation and cryopreservation of embryonic axes of peach.

Author(s) : BOUCAUD M. T. de, HELLIOT B., BRISON M.

Type of article: Article

Summary

Seeds of peach (Prunus persica L. Batsch) exhibited tolerance to dehydration down to 4.9% moisture content (dry weight basis) but were unable to germinate after exposure to liquid nitrogen. Therefore excised embryonic axes were used for cryopreservation. Two cryopreservation procedures were compared: desiccation to 7.4% moisture content followed by rapid freezing or a 1 hour dehydration period followed by the application of a cryoprotective solution, either 1,2-propanediol-sucrose or modified PVS2, for a period varying from 20 to 90 minutes and rapid freezing. Both cryopreservation procedures being as efficient (until 85-95% regrowth rate), it is advised to use the simpler desiccation procedure.

Details

  • Original title: Desiccation and cryopreservation of embryonic axes of peach.
  • Record ID : 1997-2906
  • Languages: English
  • Source: Cryo-Letters - vol. 17 - n. 6
  • Publication date: 1996/11
  • Document available for consultation in the library of the IIR headquarters only.

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