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Design of transgenic cell cultures as model systems for cryopreservation research.

Author(s) : SCHUMACHER H. M., BANNA A. el, ALI Z., et al.

Summary

In the present approach a viral IRES element has been used to construct dicistronic vectors co-expressing a target and a reporter gene. From these vectors a unique mRNA is transcribed. During translation the IRES element, acting as stop codon, leads to cap-independent recruitment of ribosomes. Thus two physically independent proteins are formed. The reporter gene luc is used as second cistron and target genes providing traits relevant for cryopreservation as first cistrons. The constructed vectors were tested for expression monitoring in cell cultures.

Details

  • Original title: Design of transgenic cell cultures as model systems for cryopreservation research.
  • Record ID : 30005414
  • Languages: English
  • Source: Cryopreservation of crop species in Europe. COST Action 871. Proceedings of the final meeting, Angers, France, February 08-11, 2011.
  • Publication date: 2011/02/08

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