Share

Development of a cryopreservation process for embryogenic calluses of a commercial hybrid of sugarcane (Saccharum Sp.) and application to different varieties.

Author(s) : EKSOMTRAMAGE T., PAULET F., GUIDERDONI E., et al.

Type of article: Article

Summary

An efficient cryopreservation technique was set up with embryogenic calluses of sugarcane. Up to 92% regrowth was obtained from cryopreserved calluses of one variety after pregrowth with 0.3 M sucrose and 15% DMSO, freezing at 0.5 deg C/minute to -45 deg C and rapid thawing in a water-bath at 40 deg C. Numerous plantlets, comparable to that coming from control calluses, could be regenerated and grown in the greenhouse. This technique was successfully applied to calluses of 10 varieties out of 11 tested during three successive experiments.

Details

  • Original title: Development of a cryopreservation process for embryogenic calluses of a commercial hybrid of sugarcane (Saccharum Sp.) and application to different varieties.
  • Record ID : 1993-0263
  • Languages: English
  • Source: Cryo-Letters - vol. 13 - n. 4
  • Publication date: 1992/07
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (4)
See the source

Indexing