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Cryopreservation of encapsulated apices of sugarcane: effect of freezing procedure and histology.

Author(s) : ARNAO M. T. G., ENGELMANN F., HUET C., URRA C.

Type of article: Article

Summary

The effect of sucrose concentration during the pregrowth treatment and of the freezing procedure on the survival of encapsulated apices of 6 sugarcane varieties was investigated. The optimal sucrose concentration was 0.75 M. Survival was noted with both rapid freezing by direct immersion in liquid nitrogen and slow freezing using a programmable freezer but it was generally higher after rapid cooling. Important genotypic variation was observed for both parameters studied.

Details

  • Original title: Cryopreservation of encapsulated apices of sugarcane: effect of freezing procedure and histology.
  • Record ID : 1994-1622
  • Languages: English
  • Source: Cryo-Letters - vol. 14 - n. 5
  • Publication date: 1993/09
  • Document available for consultation in the library of the IIR headquarters only.

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