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Direct regrowth of encapsulated somatic embryos of coffee (Coffea canephora) after cooling in liquid nitrogen.

Author(s) : HATANAKA T., YASUDA T., YAMAGUCHI T., SAKAI A.

Type of article: Article

Summary

Somatic embryos of Coffea canephora were successfully cryopreserved after being exposed to a series of preparative steps: 1) somatic embryos were progressively precultured on media with increasing concentrations of sucrose (0.3, 0.5, 0.7 and 0.8 M), growing for 3-4 days on each medium; 2) they were then encapsulated in alginate beads that contained 0.5 M sucrose; 3) these beads were dehydrated up to about 13% and plunged into liquid nitrogen. 63% of cryopreserved embryos remained alive and about 50% of them grew directly without formation of secondary embryos and calluses. The remaining meristems from cryopreserved material ceased to grow after rooting. This cryogenic procedure appears to be a promising technique for cryopreservation of coffee somatic embryos.

Details

  • Original title: Direct regrowth of encapsulated somatic embryos of coffee (Coffea canephora) after cooling in liquid nitrogen.
  • Record ID : 1994-2305
  • Languages: English
  • Source: Cryo-Letters - vol. 15 - n. 1
  • Publication date: 1994/01
  • Document available for consultation in the library of the IIR headquarters only.

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