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DNA SYNTHESIS IN ORGAN CULTURES OF CRYOPRESERVED HUMAN SKIN.

[In Russian. / En russe.]

Author(s) : VOLKOVA N. A., HVOSTOVA S. G., SANDOMIRSKIJ B. P.

Type of article: Article

Summary

THE ABILITY OF CADAVER SKIN PIECES TAKEN UPON PREPARATION, EQUILIBRATION WITH A CRYOPROTECTIVE MEDIUM AND AFTER THAWING TO INCORPORATE A LABELED PRECURSOR OF DNA SYNTHESIS 3 H-TYMIDINE IN ORGAN CULTURES WAS STUDIED. EXPOSURE OF CADAVER SKIN PIECES TO A CRYOPROTECTIVE MEDIUM CONTAINING 15% PEO-400 FOR 3 HR APPEARED TO INHIBIT MARKEDLY THE INCORPORATION OF 3 H-TYMIDINE INTO PROCESSES OF EPIDERMOCYTE PROLIFERATION. USING 3 H-TYMIDINE LABELS IN CULTURES OF THAWED SKIN SAMPLES REVEALED PROLIFERATION OF BASAL EPIDERMOCYTES OF HAIRY FOLLICULI.

Details

  • Original title: [In Russian. / En russe.]
  • Record ID : 1987-1599
  • Languages: Russian
  • Source: Kriobiologija - n. 2
  • Publication date: 1986
  • Document available for consultation in the library of the IIR headquarters only.

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