ShareEffect of cryoprotective additives and cryopreservation protocol on Plasmodium berghei.
Author(s) : POPESCU C., STERIU D., DAVID C.
Type of article: Article
Summary
Optimal cryoprotection conditions for Plasmodium berghei, a parasitic malaria strain found in mice, were investigated. About 90% cell survival was obtained with continuous slow cooling of 1 deg C/minute from 20 to 0 deg C and 3 deg C/minute to -40 deg C followed by immersion in liquid nitrogen with some combinations of DMSO, L-glutamine and sorbitol in RPMI 1640 medium and fast thawing at 35 deg C.
Details
- Original title: Effect of cryoprotective additives and cryopreservation protocol on Plasmodium berghei.
- Record ID : 1996-3744
- Languages: English
- Source: Cryo-Letters - vol. 16 - n. 6
- Publication date: 1995/11
- Document available for consultation in the library of the IIR headquarters only.
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CRYOPRESERVATION OF GIARDIA LAMBLIA WITH DIMETH...
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- Date : 1984
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- Source: Cryobiology - vol. 21 - n. 2
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- Date : 1989/03
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- Source: Cryo-Letters - vol. 10 - n. 2
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- Source: Proceedings of the 25th IIR International Congress of Refrigeration: Montréal , Canada, August 24-30, 2019.
- Formats : PDF
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Cryopreservation of living organs.
- Author(s) : TANASAWA I., NAGATA S., KIMURA N.
- Date : 1993
- Languages : Japanese
- Source: Trans. JAR - vol. 10 - n. 1
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A simple technique for cryopreservation of the ...
- Author(s) : MARCIN A., et al.
- Date : 1992/05
- Languages : English
- Source: Cryo-Letters - vol. 13 - n. 3
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