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EFFECT OF CRYOPROTECTIVE DILUENT AND METHOD OF FREEZE-THAWING ON SURVIVAL AND ACROSOMAL INTEGRITY OF RAM SPERMATOZOA.

Author(s) : PONTBRIAND D.

Type of article: Article

Summary

A MULTIFACTORIAL STUDY ANALYZED THE EFFECTS OF FREEZING METHOD, CRYOPROTECTIVE DILUENT, SEMEN TO DILUENT RATIO, AND THAWING VELOCITY ON POST-THAW MOTILITY, PROGRESSIVE STATUS, AND ACROSOMAL INTEGRITY OF RAM SPERMATOZOA. SPERMATOZOAL VIABILITY WAS HIGHLY DEPENDENT ON FREEZING METHOD AND CRYOPROTECTANT. IMPROVED RESULTS WERE OBTAINED BY FREEZING SEMEN IN 0.5-MILLILITER FRENCH STRAWS. OF THE 2 CRYOPROTECTIVE DILUENTS TESTED, BF5F YIELDED ABOUT 50% FEWER SPERMATOZOA WITH LOOSE ACROSOMAL CAPS COMPARED TO TEST. THE RESULTS SUGGEST THAT THE POOR FERTILITY RATES OFTEN EXPERIENCED USING THAWED RAM SEMEN LIKELY RESULT FROM A COMPROMISED ULTRASTRUCTURAL INTEGRITY.

Details

  • Original title: EFFECT OF CRYOPROTECTIVE DILUENT AND METHOD OF FREEZE-THAWING ON SURVIVAL AND ACROSOMAL INTEGRITY OF RAM SPERMATOZOA.
  • Record ID : 1990-1709
  • Languages: English
  • Source: Cryobiology - vol. 26 - n. 4
  • Publication date: 1989

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