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THE EFFECT OF GLYCEROL CONCENTRATION AND COOLING VELOCITY ON CRYOSURVIVAL OF RAM SPERMATOZOA FROZEN IN STRAWS.

Author(s) : FISER P. S., FAIRFULL R. W.

Type of article: Article

Summary

THE BEST SURVIVAL RATES WERE OBTAINED WHEN THE GLYCEROL CONCENTRATION WAS 4 OR 6% AND WHEN THE RATE OF FREEZING RANGED FROM 10 TO 100 K/MIN. NO SPERMATOZOA SURVIVED IF STRAWS ARE PLUNGED INTO LIQUID NITROGEN. THE RANGE OF COOLING RATES SHIFTS TO LOWER VALUES AS THE GLYCEROL CONCENTRATION INCREASES FOR OPTIMUM CRYOSURVIVAL. THE TOXIC EFFECT OF INCREASING THE CONCENTRATION OF GLYCEROL OVER 8% CONTRIBUTES GREATLY TO THE GRADUAL DECREASE IN CRYOSURVIVAL OF SPERMATOZOA AT THESE PARTICULAR CONCENTRATIONS.

Details

  • Original title: THE EFFECT OF GLYCEROL CONCENTRATION AND COOLING VELOCITY ON CRYOSURVIVAL OF RAM SPERMATOZOA FROZEN IN STRAWS.
  • Record ID : 1985-1317
  • Languages: English
  • Source: Cryobiology - vol. 21 - n. 5
  • Publication date: 1984

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