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Effect of electroporation on intracellular ice formation in rye leaf protoplasts.

Author(s) : CHANDRASEKARAN M., PITT R. E., ANESHANSLEY D. J., PARKS J. E.

Type of article: Article

Summary

Treatment with the weakest field strength and capacitance caused the median intracellular ice formation temperature to be shifted to lower temperatures relative to the control during subsequent cooling in isotonic saline. As field strength or capacitance increased, treatments resulted in more rapid intracellular ice formation kinetics and higher median intracellular ice formation temperatures relative to untreated protoplasts. The effects of electrical treatment were suppressed in hypotonic saline and were comparable in linear cooling and isothermal protocols.

Details

  • Original title: Effect of electroporation on intracellular ice formation in rye leaf protoplasts.
  • Record ID : 1996-1618
  • Languages: English
  • Source: Cryobiology - vol. 32 - n. 5
  • Publication date: 1995/10

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