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EFFECT OF THE COOLING RATE ON THE VIABILITY OF BONE MARROW HEMATOPOIETIC CELLS.

[In Chinese. / En chinois.]

Author(s) : LIU Z. B.

Type of article: Article

Summary

THE EFFECT OF COOLING RATE ON THE VIABILITY OF BONE MARROW NUCLEATED CELLS AND PLURIPOTENT STEM CELL (CFU-S) WAS STUDIED IN MICE BY MEANS OF DYE-EXCLUSION TEST, CELL VOLUME DISTRIBUTION STUDY AND CFU-S ASSAY TECHNIQUE. WHEN THE CELL SUSPENSION WAS COOLED TO 1.13, 1.75, AND 4.22K/MIN, THE SURVIVAL RATE OF CFU-S DECREASED TO 76.3, 27.7, AND 17.0% RESPECTIVELY. SOME PROBLEMS ABOUT THE TECHNIQUE OF CRYOPRESERVATION OF BONE MARROW AND THE METHOD ASSAYING THE VIABILITY OF FROZEN AND THAWED HEMATOPOIETIC CELLS ARE DISCUSSED.

Details

  • Original title: [In Chinese. / En chinois.]
  • Record ID : 1982-1759
  • Languages: Chinese
  • Source: J. chin. Assoc. Refrig. - n. 3
  • Publication date: 1981/09
  • Document available for consultation in the library of the IIR headquarters only.

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