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IIR document

Effects of ice seeding temperature on intracellular freezing of cells.

Number: pap. n. 372

Author(s) : WANG Y., ZHU K.

Summary

When the cell suspension were cooled to a certain degree of supercooling and maintained at this temperature for a few minutes, a certain amount of cells exhibited intracellular ice. The onset of intracellular ice formation did not happened simultaneously. The cells exhibited intracellular ice freezing one after another. There existed an elapsed time for cells to freeze intracellularly at constant temperature. In isotonic solution, the elapsed time of IIF was 5.4s and 39.5s with 3.4 and 11.4°C supercooling; in 0.52M glycerol, the corresponding elapsed time is less than 1s and 13s. It took a longer elapsed time for ice forming in cells when the ice seeded at lower temperature. This phenomenon represent a new way to investigate the mechanism of intracellular ice formation.

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Pages: 5 p.

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Details

  • Original title: Effects of ice seeding temperature on intracellular freezing of cells.
  • Record ID : 30016253
  • Languages: English
  • Source: Proceedings of the 24th IIR International Congress of Refrigeration: Yokohama, Japan, August 16-22, 2015.
  • Publication date: 2015/08/16
  • DOI: http://dx.doi.org/10.18462/iir.icr.2015.0372

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