Share
IIR documentSummary
When the cell suspension were cooled to a certain degree of supercooling and maintained at this temperature for a few minutes, a certain amount of cells exhibited intracellular ice. The onset of intracellular ice formation did not happened simultaneously. The cells exhibited intracellular ice freezing one after another. There existed an elapsed time for cells to freeze intracellularly at constant temperature. In isotonic solution, the elapsed time of IIF was 5.4s and 39.5s with 3.4 and 11.4°C supercooling; in 0.52M glycerol, the corresponding elapsed time is less than 1s and 13s. It took a longer elapsed time for ice forming in cells when the ice seeded at lower temperature. This phenomenon represent a new way to investigate the mechanism of intracellular ice formation.
Available documents
Format PDF
Pages: 5 p.
Available
Public price
20 €
Member price*
Free
* Best rate depending on membership category (see the detailed benefits of individual and corporate memberships).
Details
- Original title: Effects of ice seeding temperature on intracellular freezing of cells.
- Record ID : 30016253
- Languages: English
- Source: Proceedings of the 24th IIR International Congress of Refrigeration: Yokohama, Japan, August 16-22, 2015.
- Publication date: 2015/08/16
- DOI: http://dx.doi.org/10.18462/iir.icr.2015.0372
Links
See other articles from the proceedings (657)
See the conference proceedings
Indexing
-
ISOTHERMAL NUCLEATION STAGE AND ITS APPLICATION...
- Author(s) : HUBEL A., TONER M., CRAVALHO E. G.
- Date : 1987/08/24
- Languages : English
- Source: Development in refrigeration, refrigeration for development. Proceedings of the XVIIth international Congress of Refrigeration.
- Formats : PDF
View record
-
CRYOBIOLOGY OF REPRODUCTIVE CELLS.
- Author(s) : GRISCENKO V. I.
- Date : 1987/08/02
- Languages : Russian
View record
-
Effect of gap-junctional cell-to-cell communica...
- Author(s) : FUKUNAGA T., KUBO H., KURATA K., et al.
- Date : 2015/08/16
- Languages : English
- Source: Proceedings of the 24th IIR International Congress of Refrigeration: Yokohama, Japan, August 16-22, 2015.
- Formats : PDF
View record
-
VISUALIZATION OF FREEZING DAMAGE TO BIOLOGICAL ...
- Author(s) : ENGLICH S., KORBER C., RAU G.
- Date : 1987/08/24
- Languages : English
- Source: Development in refrigeration, refrigeration for development. Proceedings of the XVIIth international Congress of Refrigeration.
- Formats : PDF
View record
-
How long do we need to store cryopreserved cell...
- Author(s) : MERICKA P., STRAKOVA H., LÁNSKÁ M., et al.
- Date : 2017/05/15
- Languages : English
- Source: Cryogenics 2017. Proceedings of the 14th IIR International Conference: Dresden, Germany, Mai 15-19, 2017.
- Formats : PDF
View record