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FACTORS AFFECTING THE SURVIVAL OF MOUSE EMBRYOS CRYOPRESERVED BY VITRIFICATION.

Author(s) : RALL W. F.

Type of article: Article

Summary

PREIMPLANTATION STAGE MOUSE EMBRYOS HAVE BEEN USED TO EXAMINE THE RESPONSE OF A SIMPLE MULTICELLULAR SYSTEM TO CRYOPRESERVATION BY THE COMPLETE VITRIFICATION OF THE SUSPENSION. FACTORS THAT INFLUENCE THE SURVIVAL OF EMBRYOS INCLUDE THE CONCENTRATION AND COMPOSITION OF THE VITRIFICATION SOLUTION, THE PROCEDURE USED TO EQUILIBRATE EMBRYOS IN THIS SOLUTION, THE COOLING AND WARMING CONDITIONS, AND THE PROCEDURE USED TO DILUTE EMBRYOS FROM THE VITRIFICATION SOLUTION. HIGH RATES OF SURVIVAL ARE OBTAINED. FULL PERMEATION OF CRYOPROTECTANTS INTO THE CELLS IS NOT NECESSARY AS PARTIAL PERMEATION AND OSMOTIC SHRINKAGE CONCENTRATES THE ENDOGENOUS CYTOPLASMIC MACROMOLECULES AND GREATLY INCREASES THE LIKELIHOOD OF INTRACELLULAR VITRIFICATION.

Details

  • Original title: FACTORS AFFECTING THE SURVIVAL OF MOUSE EMBRYOS CRYOPRESERVED BY VITRIFICATION.
  • Record ID : 1988-0832
  • Languages: English
  • Source: Cryobiology - vol. 24 - n. 5
  • Publication date: 1987

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