Share

SURVIVAL IN VITRO OF ZONA PELLUCIDA-FREE MOUSE EMBRYOS AFTER COOLING BY CONVENTIONAL TWO-STEP OR VITRIFICATION METHODS.

Author(s) : BIELANSKI A.

Type of article: Article

Summary

MORULA AND BLASTOCYST STAGE ZONA PELLUCIDA (ZP)-INTACT AND ZP-FREE MOUSE EMBRYOS WERE CRYOPRESERVED BY THE CONVENTIONAL TWO STEP SLOW COOLING METHOD AND BY VITRIFICATION. RAPID WARMING AND REMOVALOF CRYOPROTECTANT BY A 1.0 M SUCROSE SOLUTION WERE USED WITH BOTH COOLING METHODS. A GREATER DECREASE IN THE VIABILITY OF ZP-FREE THAN ZP-INTACT EMBRYOS WAS OBSERVED WITHIN THE FIRST 3 HRS POST WARMING REGARDLESS OF THE STAGE OF DEVELOPMENT OF EMBRYOS AND THE METHOD OF COOLING. TOTAL SURVIVAL AT 24 HRS POST THAW FOR ZP-FREE EMBRYOS FOLLOWING VITRIFICATION WAS 40% IN CONTRAST TO 25% FOR ZP-FREE EMBRYOS FROZEN BY THE CONVENTIONAL TWO STEP METHOD.

Details

  • Original title: SURVIVAL IN VITRO OF ZONA PELLUCIDA-FREE MOUSE EMBRYOS AFTER COOLING BY CONVENTIONAL TWO-STEP OR VITRIFICATION METHODS.
  • Record ID : 1988-0831
  • Languages: English
  • Source: Cryo-Letters - vol. 8 - n. 5
  • Publication date: 1987
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (5)
See the source

Indexing