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FREE FATTY ACID ACCUMULATION FOLLOWING COLD ISCHAEMIA IN RABBIT KIDNEYS AND THE INVOLVEMENT OF A CALCIUM DEPENDENT PHOSPHOLIPASE A2.

Author(s) : COTTERILL L. A.

Type of article: Article

Summary

RABBIT KIDNEYS WERE HARVESTED AND IMMEDIATELY FLUSHED WITH 30 MILLILITERS OFCOLD HYPERTONIC CITRATE SOLUTION AND STORED FOR 72 HOURS AT 273 K (0 DEG C). THEY WERE THEN DISSECTED INTO CORTEX AND MEDULLA, IMMEDIATELY FROZEN IN LIQUID NITROGEN AND FREE FATTY ACIDS WERE EXTRACTED. THIS PERIOD OF STORAGE RESULTED IN SIGNIFICANT INCREASES IN FREE UNSATURATED FATTY ACIDS IN BOTH CORTEX AND MEDULLA. THE DATA PROVIDE SOME EVIDENCE FOR THE INVOLVEMENT OF CALCIUM ACTIVATION OF PHOSPHOLIPASE A2 IN RELEASING ARACHIDONIC ACID FROM CELLULAR MEMBRANES DURING STORAGE.

Details

  • Original title: FREE FATTY ACID ACCUMULATION FOLLOWING COLD ISCHAEMIA IN RABBIT KIDNEYS AND THE INVOLVEMENT OF A CALCIUM DEPENDENT PHOSPHOLIPASE A2.
  • Record ID : 1990-2147
  • Languages: English
  • Source: Cryo-Letters - vol. 10 - n. 6
  • Publication date: 1989/11
  • Document available for consultation in the library of the IIR headquarters only.

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