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EFFECT OF COOLING AND WARMING RATE ON GLYCEROLIZED RABBIT KIDNEYS.

Author(s) : JACOBSEN I. A.

Type of article: Article

Summary

RABBIT KIDNEYS, PERMEATED WITH 2 M GLYCEROL WERE COOLED TO 193 K (-80 DEG C) AT FOUR RATES VARYING FROM 1 K/HR TO 3.1 K/MIN AND THEN REWARMED AT FOUR RATES FROM 1 K/HR TO 4.2 K/MIN. AFTER GRADUAL DEGLYCEROLIZATION AT 283 K (10 DEG C), EACH KIDNEY WAS AUTOGRAFTED AND OBSERVED FOR 30 MIN. THE BEST RESULTS WERE OBTAINED AFTER COOLING AT 1 K/HR ; WARMING RATE HAD LITTLE INFLUENCE BUT A WARMING RATE AROUND 1 K/MIN IS PREFERRED. HISTOLOGICAL STUDIES REVEALED EXTENSIVE VASCULAR DAMAGE. FREEZE SUBSTITUTION, CARRIED OUT ON SAMPLES REMOVED AT 193 K, DEMONSTRATED EXTENSIVE ICE FORMATION IN THE INTERSTITIAL SPACE. INTRACAPILLARY ICE FORMATION WAS REDUCED ON COOLING AT 1 K/HR.

Details

  • Original title: EFFECT OF COOLING AND WARMING RATE ON GLYCEROLIZED RABBIT KIDNEYS.
  • Record ID : 1985-2232
  • Languages: English
  • Source: Cryobiology - vol. 21 - n. 6
  • Publication date: 1984

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