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Cryopreservation of the mammalian kidney. 1. Transplantation of rabbit kidneys perfused with EC (Euro-Collins) and RPS-2 (renal preservation solution 2) at 2-4 deg C.

Author(s) : KHIRABADI B. S., FAHY G. M.

Type of article: Article

Summary

The authors evaluated RPS-2 in comparison to EC solution using a modified technique for rabbit kidney autotransplantation and a computer-based organ perfusion machine designed for the introduction and removal of cryoprotective agents. Consistent success in rabbit kidney transplantation was found to depend on the anesthetic used, the hydration volumes administered, and direct ureter-to-ureter anastomosis. RPS-2 was found to be equivalent to EC for short-term (about 5 h) preservation by either perfusion or simple cold storage. However, good results with EC were associated with perfusion at 4 deg C, recovery being significantly worse at 2 deg C. These results establish perfusates, perfusion conditions, transplantation techniques, computer-based perfusion control techniques, and a general clinical baseline that are permissive of further direct experiments on cryoprotectant introduction and removal.

Details

  • Original title: Cryopreservation of the mammalian kidney. 1. Transplantation of rabbit kidneys perfused with EC (Euro-Collins) and RPS-2 (renal preservation solution 2) at 2-4 deg C.
  • Record ID : 1995-1222
  • Languages: English
  • Source: Cryobiology - vol. 31 - n. 1
  • Publication date: 1994/02

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