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FREEZE DENATURATION OF ENZYMES AND ITS PREVENTION WITH ADDITIVES.

Author(s) : TAMIYA T.

Type of article: Article

Summary

FREEZE INACTIVATION OF LDH, MDH, ADH, G-6-PDH AND PK (LACTATE DEHYDROGENASE, MALATE DEHYDROGENASE, ALCOHOL-DEHYDROGENASE, GLUCOSE-6-PHOSPHODEHYDROGENASE AND PYRUVATE KINASE)AND ITS PREVENTION WITH ADDITIVES SUCH AS SODIUM GLUTAMATE AND ALBUMIN WERE STUDIED. LDH, MDH, ADH, G-6-PDH, AND PK, EACH LOST THEIR ACTIVITY DURING FROZEN STORAGE AT 253 K (-20 DEG C). THE SPEED OF THE INACTIVATION DIFFERED IN EACH. THE STABILITY OF THE ENZYMES INCREASED WITH THE INCREASE OF THE ENZYME CONCENTRATION. SODIUM GLUTAMATE AND ALBUMIN PREVENTED THE FREEZE INACTIVATION. COMBINED USE OF GLUTAMATE AND ALBUMIN PREVENTED THE INACTIVATION THE BEST AND MAINTAINED THE INITIAL ACTIVITY ALMOST COMPLETELY OVER 6 WEEKS. THE INACTIVATION OF THE ENZYMES WAS LOST PARTIALLY WHEN THEIR SOLUTIONS WERE DILUTED AND PREVENTED BY ADDITION OF SODIUM GLUTAMATE AND/OR ALBUMIN.

Details

  • Original title: FREEZE DENATURATION OF ENZYMES AND ITS PREVENTION WITH ADDITIVES.
  • Record ID : 1986-2102
  • Languages: English
  • Source: Cryobiology - vol. 22 - n. 5
  • Publication date: 1985

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