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FREEZING OF BOVINE EMBRYOS.

CONGELATION D'EMBRYONS DE BOVINS.

Type of article: Periodical article

Summary

DESCRIPTION OF THE TECHNIQUE OF THE CENTRE DE GENETIQUE ANIMALE (INRA/FRANCE): COLLECTION OF EMBRYOS AT THE BLASTOCYTE STAGE, 7 DAYS AFTER FERTILISATION ; ADDITION OF A CRYOPROTECTANT (GLYCEROL SOLUTION AT 10%) ; PLACING IN PLASTIC STRAWS ; FREEZING TO 266 K (-7 DEG C), THEN TO 238 K (-35 DEG C) AT 0.3 K/MIN AND KEEPING IN LIQUID NITROGEN FOR SEVERAL MONTHS. THE EMBRYO IS THAWED IN A FEW SECONDS AND INTRODUCED IN THE UTERUS OF THE RECEPTOR COW. THE FIRST RESULTS SHOW A YIELD OF ABOUT 50% (RATIO OF THE NUMBER OF CALVES TO THE NUMBER OF FROZEN EMBRYOS). G.G.

Details

  • Original title: CONGELATION D'EMBRYONS DE BOVINS.
  • Record ID : 1983-1180
  • Languages: French
  • Source: Vet. fr. - n. 2
  • Publication date: 1982
  • Document available for consultation in the library of the IIR headquarters only.

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