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HYDRAULIC PERMEABILITY AND ACTIVATION ENERGY OF HUMAN KERATINOCYTES AT SUBZERO TEMPERATURES.

Author(s) : AGGARWAL S. J., DILLER K. R., BAXTER C. R.

Type of article: Article

Summary

STUDIES ON ISOLATED HUMAN KERATINOCYTES PROVIDE A MODEL FOR DESIGN OF OPTIMAL FREEZE-THAW PROTOCOLS FOR SKIN CRYOPRESERVATION AND BANKING. THE KEDEM-KATCHALSKY COUPLED FLOW TRANSPORT MODEL WAS STATISTICALLY FIT TO THE DATA USING A PERSONAL COMPUTER. VALUES FOR THE PERMEABILITY COEFFICIENTS WERE ADJUSTED TO OPTIMIZE THE CORRELATION BETWEEN THE THEORY AND THE DATA. AN ACTIVATION ENERGY OF 44.8 KILOJOULES/MOL AND A WATER PERMEABILITY OF 20. 725 PICOMETERS/PASCAL.HOUR AT 273 K (0 DEG C) WERE DERIVED FROM THE DATA MEASURED OVER A TEMPERATURE RANGE FROM 271 TO 264 K (-2 TO -9 DEG C).

Details

  • Original title: HYDRAULIC PERMEABILITY AND ACTIVATION ENERGY OF HUMAN KERATINOCYTES AT SUBZERO TEMPERATURES.
  • Record ID : 1989-0787
  • Languages: English
  • Source: Cryobiology - vol. 25 - n. 3
  • Publication date: 1988

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