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Ice-nucleating bacteria from the guts of two sub-Antarctic beetles Hydromedion sparsutum and Perimylops antarcticus (Perimylopidae).

Author(s) : WORLAND M. R., BLOCK W.

Type of article: Article

Summary

The site of ice nucleation in the freeze-tolerant, sub-Antarctic beetle Hydromedion sparsutum has been investigated. Ice(+) bacteria, active at above -2°C, were isolated from the guts of beetles and identified as a fluorescent Pseudomonas species. Other possible sites of nucleation, including the hemolymph, were examined but had a lower activity. Ice(+) bacteria were isolated from mixed populations, and grown on nutrient agar plates and in nutrient broth. Nucleation activity of the broth culture peaked after only 2 days although the number of live cells continued to increase until day 6. These cultures were used to determine the maximum nucleation activity of a bacterial suspension in sterile distilled water (-3.4°C) and the dilution factor required to cause a 50% reduction in activity (10,000).

Details

  • Original title: Ice-nucleating bacteria from the guts of two sub-Antarctic beetles Hydromedion sparsutum and Perimylops antarcticus (Perimylopidae).
  • Record ID : 2000-0444
  • Languages: English
  • Source: Cryobiology - vol. 38 - n. 1
  • Publication date: 1999/01

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